Bolsa de Investigação (M/F) (19-01-10) - IBMC

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Bolsa de Investigação (M/F) (19-01-10)
IBMC

Referência: BioModularH2 (FP6-NEST-2005-Path-SYN-043340)

Título do Projecto: Engineered Modular Bacterial Photoproduction of Hydrogen
Código interno: PR011703

Está aberto concurso para recrutamento de um(a) bolseiro(a) de Investigação para colaborar no projecto acima referido, financiado pela União europeia.

A bolsa, em regime de exclusividade, terá a duração de 5 meses, com início previsto em 15 de Fevereiro de 2010.

O valor mensal da bolsa será de € 745,00, pago por transferência bancária (preferencialmente).

Local de trabalho: Unidade de Investigação de Microbiologia Celular Aplicada, IBMC, Porto.

Programa de trabalho: Caracterização de partes e módulos sintéticos (ver sumário em anexo).

Perfil pretendido:

Os candidatos devem possuir licenciatura na área das Ciências Biológicas, Bioquímica ou afins. Dá-se preferência a candidatos com experiência em biologia molecular de cianobactérias.

O prazo para recepção de candidaturas decorre de 19 de Janeiro a 2 de Fevereiro de 2010.

As propostas deverão incluir CV e uma carta de referência e ser enviadas para o e-mail candidaturas@bmc.up.pt com indicação do código interno.

Após avaliação do CV, os candidatos pré-seleccionados poderão ser chamados para entrevista.

A contratação será regida pelo estipulado na legislação em vigor relativamente ao Estatuto de Bolseiro de Investigação Cientifica, nomeadamente a Lei 40/2004, de 18 Agosto, e o Regulamento de Bolsas de Investigação Científica do IBMC (www.ibmc.up.pt/fellowships.php).



Engineered Modular Bacterial Photoproduction of Hydrogen
BioModularH2

Supervisor/co-supervisor:
Paula Tamagnini/Catarina Pacheco
Cellular and Applied Microbiology Unit

IBMC - Institute for Molecular and Cell Biology
Rua Campo Alegre, 823
4150-180 Porto
Portugal

Project Summary:
Our project aims at designing reusable, standardised molecular building blocks that will produce a
photosynthetic bacterium containing engineered chemical pathways for competitive, clean and
sustainable hydrogen production. Our engineering approach will provide the next generation of
synthetic biology engineers with the toolbox to design complex circuits of high potential industrial
applications such as the photo-production or photo-degradation of chemical compounds with a
very high level of integration. For this purpose we have targeted on a cyanobacterium, a very
chemically rich and versatile organism highly suitable for modelling, to be used as future platform
for hydrogen production and biosolar applications. In particular, our synthetic biological approach
aims at creating an anaerobic environment within the cell for an optimized, highly active iron-only
hydrogenase by using an oxygen consuming device, which is connected to an oxygen sensing device
and regulated by artificial circuits.

This project will also help to establish a systematic hierarchical engineering methodology (parts,
devices and systems) to design artificial bacterial systems using a truly interdisciplinary approach
that decouples design from fabrication. We aim to construct biological molecular parts by
engineering proteins with new enzymatic activities and molecular recognition patterns, by
combining computational and in vitro evolution methodologies. Subsequently, we will design novel
devices (e.g. input/output, regulatory and metabolic) by combining these parts and by using the
emerging knowledge from systems biology. Furthermore, we shall design custom circuits of devices
applying control engineering and optimisation. In parallel, we will develop a cyanobacterial
“chassis” able to integrate our synthetic circuits using a model-driven biotechnology.